FIG. 5.

Determination of voltage-dependent compliance change by the sinusoidal method. a Voltage command waveform and fiber motion command waveform as a function of time. The fiber sinusoid amplitude was 300 nm. The voltage command sinusoid amplitude was usually 120 mV. The step voltage command was varied. b Typical fiber motion waveform (cell extension upward) in response to the above fiber and voltage commands. This cell was 35 μm long, and the biasing step voltage command was 240 mV. The calculated compliance was 0.20 km/N before and 0.23 km/N during the bias voltage command. c Fractional compliance change as a function of estimated membrane potential change for 32 measurements from 26 cells (all cells studied from six animals). The solid line represents the expected fractional compliance change if the cell behaved like the reference cell. The line fitted to the data has a slope of 0.00094 and an intercept of 0.044. d Correlation between compliance measures for the same cells (12 cells). The fitted line has a slope of 1.20 and an intercept of 0.013 (R = 0.962).