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NGF mRNA levels were measured by real-time PCR in hindpaw skin (panel A) and proximal tibia (panel B) in control and fracture rats. At 4 weeks after fracture NGF expression was increased in the hindpaw skin (n = 16 per cohort) and in the bone (n = 8 per cohort). ***P < 0.001 for fracture vs. control values.
