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Subcellular distribution of Chs6p. 7MYC-CHS6 (Z136) cells were grown to midlog phase and processed by Con A fractionation. The membranes in the 10,000 × g supernatant fraction were adjusted to 40% (wt/vol) Nycodenz and floated through a layer of 35% (wt/vol) Nycodenz. T, P, and S indicate the total, 10,000 × g pellet, and supernatant fractions, respectively. L indicates the load sample for the flotation gradient, and 1–4 represent 100 μl fractions from the top of the gradient.
