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HEK293-produced human IFNα2b is biologically active. The biological activity of HEK293-produced human IFNα2b was assayed with a gene reporter assay and compared to E. coli-produced human recombinant IFNα2b as described in material and methods. The activity of the secreted alkaline phosphatase is plotted against the concentration of IFNα2b produced in the two hosts. Each point represents the average ± SEM of 3 experiments performed in triplicate.
