Table 6.
Purpose | Gene | Gene segment specification (according Figure 1) or former gene name | DNA/cDNA-derived PCR product length (bp) | Forward primers | Reverse primers |
PCR-based cloning | F3H | Segment 1 | variable | atggcgccggtgagcaac | tttacgtggcatggcatgcat |
F3H | Segment 2 | variable | atgacgcgcctctctcgcg | tggacggtgatccaggtcttg | |
F3H | Segment 3 | variable | [11] | [11] | |
F3H | Segment 4 | variable | tctcgatcgatcgaccaccaa | ctaggcaagaatttcgttgaggg | |
F3H | Segment 5 | variable | ccggtgagcaacgagacgttc | ggcaagaatttcgttgagggg | |
Chromosomal assignment and | F3H-A1 | T. aestivum F3H1 | 703/- | gccacctgcaggtatacacgcat | ccaccgcccgtagtccct |
physical mapping | F3H-B1 | T. aestivum F3H3 | 333/- | gcgtgctgtccgaggcgc | cgatcgatcgattaaggatt |
F3H-B2 | T. aestivum F3H4 | 255/155 | gctgcctgccgaggacaagg | aacgcccgtagtcccgtgcc | |
F3H-D1 | T. aestivum F3H2 | 225/- | gccacctgcaggtacccacacat | ccacctcccgtagtcccg | |
F3H-G1 | T. timopheevii F3H2t | 371/- | acgactcatggggctgtca | caattggtggtcgatcgatcag | |
qRT-PCR, RT-PCR | F3H-A1 | T. aestivum F3H1 | 800/186 | atgacacgcctctctcgcg | ccaccgcccgtagtccct |
F3H-B1 | T. aestivum F3H3 | 830/183 | tgacgcgcctctctcgcgag | accgcccgtagtcccgtgct | |
F3H-B2 | T. aestivum F3H4 | 255/155 | gctgcctgccgaggacaagg | aacgcccgtagtcccgtgcc | |
F3H-D1 | T. aestivum F3H2 | 281/145 | atcgtctccagccacctgcag | cgctgtatcgctccaccacg |