Table 6.

Primers designed to amplify wheat F3H for cloning, chromosomal localization and for expression analysis.

Purpose	Gene	Gene segment specification (according Figure 1) or former gene name	DNA/cDNA-derived PCR product length (bp)	Forward primers	Reverse primers
PCR-based cloning	F3H	Segment 1	variable	atggcgccggtgagcaac	tttacgtggcatggcatgcat
	F3H	Segment 2	variable	atgacgcgcctctctcgcg	tggacggtgatccaggtcttg
	F3H	Segment 3	variable	[11]	[11]
	F3H	Segment 4	variable	tctcgatcgatcgaccaccaa	ctaggcaagaatttcgttgaggg
	F3H	Segment 5	variable	ccggtgagcaacgagacgttc	ggcaagaatttcgttgagggg
Chromosomal assignment and	F3H-A1	T. aestivum F3H1	703/-	gccacctgcaggtatacacgcat	ccaccgcccgtagtccct
physical mapping	F3H-B1	T. aestivum F3H3	333/-	gcgtgctgtccgaggcgc	cgatcgatcgattaaggatt
	F3H-B2	T. aestivum F3H4	255/155	gctgcctgccgaggacaagg	aacgcccgtagtcccgtgcc
	F3H-D1	T. aestivum F3H2	225/-	gccacctgcaggtacccacacat	ccacctcccgtagtcccg
	F3H-G1	T. timopheevii F3H2t	371/-	acgactcatggggctgtca	caattggtggtcgatcgatcag
qRT-PCR, RT-PCR	F3H-A1	T. aestivum F3H1	800/186	atgacacgcctctctcgcg	ccaccgcccgtagtccct
	F3H-B1	T. aestivum F3H3	830/183	tgacgcgcctctctcgcgag	accgcccgtagtcccgtgct
	F3H-B2	T. aestivum F3H4	255/155	gctgcctgccgaggacaagg	aacgcccgtagtcccgtgcc
	F3H-D1	T. aestivum F3H2	281/145	atcgtctccagccacctgcag	cgctgtatcgctccaccacg