Figure 2:

Repression of human (h)MMP-9 promoter activity by wild-type (wt)-p53 and mutants p53.

CTB were cotransfected with 6 µg of −670 hMMP-9 CAT reporter plasmid and 1 µg of mutant, wt-p53 constructs or control vector. After 48 h, cells were harvested and CAT activity was assayed and normalized to the amount of protein in the cellular lysates. Three independent transfections, each run in triplicate, were performed and the results expressed as mean ± SEM. Relative promoter activities were calculated by arbitrarily setting the activity of the control as 100. Paired Student's t-test was used to compare cells transfected with p53 constructs versus control vector.