Figure 3:

Determination of the minimal promoter sequence required for p53 repression.

CTB were transfected with 3 µg of −670, −531 or −90 hMMP-9 promoter/CAT reporter plasmid and 0.5 µg wt-p53 or control vector. After 48 h, cells were harvested and CAT activity was assayed and normalized to the amount of proteins in the cellular lysates. Three independent experiments, each run in triplicate, were performed and the results expressed as mean ± SEM. Paired Student's t-test was used to compare transfected cells with wt-p53 versus control plasmids.