Figure 5:

Effects of PFT-α on gelatinases activities and on MMP-9 expression.

Half CTB were treated with a known p53 inhibitor, 20 µM PFT-α, for 48 h. The untreated cells were used as control. Cells were harvested and secreted MMP-9 (gelatinase B) and MMP-2 (gelatinase A) activities measured by the gelatin zymography assay in the culture medium (A). Expression of MMP-9 was evaluated by the western blot analysis (B). The zymogram (A) and western blot (B) scan corresponds to one experiment run in triplicate. However, three independent experiments, each run in triplicate, were performed and the results expressed as mean ± SEM. Relative MMP-9 and MMP-2 activities were calculated by arbitrarily setting the activity of the control as 100. Student's paired t-test was used to compare PFT-α treated and untreated cells.