Skip to main content

View full-text article in PMC

. 2008 Sep 26;3(9):e3273. doi: 10.1371/journal.pone.0003273

Table 1. Npl3 phosphorylation sites.

Npl3 (414 aa)	Phosphorylated Serines	Treatment	Analysis	Reference
Endogenous	Ser212,	WCE (whole cell extract)	MS	This study
	Ser224	WCE	MS-IMAC	This study, [34]
	Ser349, Ser356	WCE	MS-IMAC	(Ficarro et al, 2002)
	Ser411	³²P-in vivo labeling	2D gel	(Gilbert et al, 2001)
CK2 in vitro phosphorylated	Ser77, Ser79,	Kinase assay	MS	This study
	Ser212, Ser230,		“
	Ser328, Ser336,		“
	Ser349, Ser356		“
	Ser411		Point mutants

Phosphorylation sites identified in Npl3.

^*

Highlighted residues where phosphorylation sites detected in both the endogenous and in vitro CK2 phosphorylated Npl3.