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Treg expression of receptors for IL-2 family cytokines. Tregs and Th cells were prepared as described and stained for receptor expression. Alternatively, isolated cells were preactivated for 48 h with immobilized anti-CD3 antibody and IL-2 prior to staining for receptor expression. Gray filled, Isotype control; gray line, Th cells; black line, Tregs. Cytofluorimetric analysis for each receptor was performed at least three times.
