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FoxP3 expression after stimulation with IL-2 family cytokines. FACS-sorted Tregs from Foxp3-gfp knock-in mice were stimulated with anti-CD3 and the indicated cytokines. After 48 h cells, Tregs were analyzed for FoxP3 expression by determining GFP. Mean fluorescence intensity (MFI) is indicated in parentheses. Similar results were obtained using antibodies directed against FoxP3. Results presented are representative of five similar experiments.
