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. 2008 Jul 14;84(4):1120–1129. doi: 10.1189/jlb.0108064

Fig. 3.

Fig. 3.

The effect of LPS, simvastatin, and isoprenoid intermediates (GGPP and FPP) on Ras, Rac1, and RhoA activation. (A) U937 cells were treated with 10 μM simvastatin, simvastatin plus 30 μM GGPP, or simvastatin plus 30 μM FPP in the presence of 100 ng/ml LPS for 24 h. After the treatment, membrane protein was extracted and used for immunoblotting of Ras, Rac1, and RhoA. TLR4 was immunoblotted and served as a control. The blots presented are representative of two independent experiments with similar results. (B) U937 cells were treated with 10 μM simvastatin, simvastatin plus 30 μM GGPP, or simvastatin plus 30 μM FPP in the presence of 100 ng/ml LPS for 24 h, and the cell lysate was used in the pull-down assay to determine Ras, Rac1, and RhoA activities as described in Materials and Methods. Total Ras, Rac1, and RhoA in cell lysate were also immunoblotted.