Fig. 5.

The effect of simvastatin and GGPP on LPS-stimulated AP-1 (A) or NF-κB (B) DNA-binding activity. U937 cells were treated with 10 μM simvastatin, 30 μM GGPP, or simvastastin plus GGPP in the presence or absence of 100 ng/ml LPS for 4 h. After the treatment, nuclear protein was extracted and used for EMSA to determine AP-1 or NF-κB DNA-binding activities as described in Materials and Methods. The amount of shifted AP-1 (A) or NF-κB (B) was quantified by densitometric scanning of the shifted bands. The EMSA presented is representative of two independent experiments with similar results.