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The effect of simvastatin, LPS, or simvastatin plus LPS on p38 MAPK and JNK phosphorylation. U937 cells were treated with 10 μM simvastatin. with or without 100 ng/ml LPS for 10, 30, and 60 min. After the treatment, cellular protein was extracted and subjected to immunobloting to detect total p38, total JNK, phosphorylated p38. and phosphorylated JNK. The blot presented is representative of two independent experiments with similar results. C, control; S, simvastatin; L, LPS.
