Figure 2.

Lymphoblasts acquire a nonmotile phenotype after activation of integrin LFA-1. Lymphoblasts were allowed to bind on ICAM-1-Fc–coated dishes and stimulated for 45 min with saline solution (CONTROL), 50 nM PDBu, 10 μg/ml mAb KIM-127, or 10 mM Mg²⁺ plus 1 mM EGTA (Mg²⁺/EGTA) before filming. The movement of the lymphoblasts was recorded by time-lapse video microscopy for 0, 40, 80, 120, and 160 s. The trajectories of individual cells were analyzed, and representative migration tracks are shown.