Fig. 7.

Down-regulation of IFN-γ and CXCR3 ligands in CCR7-B16 tumors. (A) Footpad tumor cells from pLNCX2-B16- and CCR7-B16-injected mice were screened for differential expression of IFN-γ and CXCL9, -10, and -11 by performing qRT-PCR on SuperArray plates. A more complete listing of up- and down-regulated mRNAs is found in Table 1. (B) qRT-PCR of the indicated cytokines and chemokines was performed on tumor cells (before and after depletion of CD45⁺ cells by affinity microbeads). (C) In vitro assessment of CXCL10 secretion by B16 cells in response to IFN-γ stimulation was performed by activating B16 cell lines with (or without) immobilized CCL21 (1 μg/ml) for 3 h prior to stimulating with IFN-γ at the indicated concentration. The cell culture supernatants were collected 24 h later to analyze CXCL10 production by ELISA. The figure depicts one of three independent experiments with similar results.