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. 2008 Jul 14;84(4):1159–1171. doi: 10.1189/jlb.0907612

Fig. 1.

Fig. 1.

Effect of P2RX7 agonists on pCREB in human monocytic cells. (A) Isolated peripheral human blood monocytes were treated with vehicle (2.5 mM HEPES), 100 μM BzATP, or 100 ng/mL LPS for the indicated times. A representative immunoblot displaying pCREB is shown (n=3). The pCREB antibody recognizes pCREB and phosphorylated ATF-1 (pATF1); thus, it is also shown. Immunoblotting was performed with anti-Grb2 antibody as a loading control. (B) THP-1 human monocytes were treated with vehicle (2.5 mM HEPES), 100 μM PMA, 1 mM ATP, or 250 μM BzATP for 15 min. A representative immunoblot displaying pCREB/pATF1 is shown (n=3). Immunoblotting was performed with anti-Grb2 antibody as a loading control. (C) Murine RAW 264.7 macrophages were stimulated with vehicle (2.5 mM HEPES), 250 μM BzATP, or 1 μg/mL LPS for the indicated time-points. Immunoblotting was performed with anti-pCREB/pATF1 and anti-β-tubulin (loading control) antibodies. Results of the three independent experiments were combined and graphed ± sem.