Fig. 5.

Effect of an ERK1/2 kinase inhibitor on P2RX7-induced CREB phosphorylation in primary human monocytes. Isolated human peripheral blood monocytes were pretreated for 15 min with vehicle (DMSO) or 10 μM UO126 and were subsequently stimulated with vehicle (2.5 mM HEPES), 250 μM BzATP, or 10 μM PMA for the indicated time-points. A representative immunoblot displaying pCREB/pATF1 is shown. Immunoblotting was performed with an anti-β-tubulin antibody as a loading control. Analogous results were observed in two separate experiments.