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. Author manuscript; available in PMC: 2009 May 15.
Published in final edited form as: Free Radic Biol Med. 2008 Feb 26;44(10):1833–1845. doi: 10.1016/j.freeradbiomed.2008.02.007

Fig. 2.

Fig. 2

Effects of apigenin treatment on p53 expression and transcriptional activity in 22Rv1 cells. (A) Immunoblots for p53 and p21/WAF-1 using lysates from 22Rv1 cells treated with various concentrations of apigenin for the indicated time periods. The blot was stripped and reprobed with antiactin antibody to ensure equal protein loading. (B) Densitometric analysis for p53 and p21/WAF-1. (C) Transcriptional activation of p53 using p53-dependent PG-13 promoter. At 24 h posttransfection with the PG-13 luciferase plasmid, 22Rv1 cells were treated with the indicated concentrations of apigenin. Data are means±SE, n=3, repeated twice with similar results. Significantly different from control: **p<0.001. Luciferase assay was performed as described under Materials and methods.