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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Cancer Cell. 2008 Aug 12;14(2):135–145. doi: 10.1016/j.ccr.2008.07.003

Figure 3. Most Math1-Cre/PtcC/C GNPs differentiate despite loss of ptc.

Figure 3

A–F. Cerebella from 6-week-old WT (A, D) and Math1-Cre/PtcC/C mice (B, C, E, F) were stained with H&E (A–C) or with anti-Ki67 (green) to detect proliferation and anti-GABRA6 (red) to detect granule neuron differentiation (D–F). Whereas WT cerebella contain no proliferating cells (A, D), mutants exhibit extensive proliferation and differentiation, with proliferating cells either localized to the surface (B, E) or in nodules surrounded by differentiated cells (C, F). G–I. Cerebellar sections from 6-week-old WT (G) and Math1-Cre/PtcC/C (H) mice were stained with cresyl violet, and the regions indicated by arrows were laser-captured for RNA analysis. I. RNA from WT IGL (lane 1, derived from region 1 in panel G), Math1-Cre/PtcC/C IGL (lane 2, from region 2 in panel H), WT EGL (lane 3, tissue not shown) and proliferating cells at the surface of the Math1-Cre/PtcC/C cerebellum (lane 4, from region 4 in panel H) were analyzed by RT-PCR for expression of ptc and beta2-microglobulin (β2m). Note the lack of ptc expression in mutant IGL (lane 2). Scale bars: panels A–C, 300 μm; panels D–H, 30 μm.