Figure 3.

(A and C) Identification of transactivation and Res2 binding domains in Rep2. Schematic representation of deletion mutants analyzed in this study. The intact rep2⁺ gene is shown at the top. The zinc-finger motif is shaded. The amino acid numbers are shown at each protein coding region. Full-length and deletion mutants of rep2⁺ were fused to a DNA binding domain of Gal4. (D) One-hybrid and two-hybrid analysis for transcriptional activator and Res2 binding domains in Rep2. The DNA binding domain hybrid was transfected into the yeast reporter strain SFY526 together with (gray bar) or without (black bar) Res2-fused Gal4 activation domain. After growth on selective medium, each transformant was assayed for β-galactosidase activity. Black bars indicate transactivation activity, and the difference between gray and black bars reflects Res2 binding activity. Experiments were repeated four times in B and three times in D; data are presented as means ± SD.