Figure 5.

Transactivation and Res2 binding domains are required for Rep2 function in the reconstitution system. Full-length and deletion mutants of rep2⁺ were inserted into the pKT10-GAPDH vector and transfected into the LacZ reporter host cells harboring res2⁺ and cdc10⁺, which were then induced for res2⁺ and cdc10⁺ by incubating at 30°C for 16 h in SD medium containing 3% galactose and 0.2% sucrose followed by β-galactosidase assay. Experiments were repeated three times, and data are presented as means ± SD.