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. 2008 Sep 9;99(6):949–956. doi: 10.1038/sj.bjc.6604632

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Copyright © 2008 Cancer Research UK

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Immunohistochemical analysis of gastric tissues for MUC4 expression by using gastric cancer tissue microarray (TMA) slides. Tissue sections were stained for MUC4 using anti-MUC4 monoclonal antibody followed by biotinylated secondary antibody incubation and streptavidin peroxidase 3,3′-diaminobenzidine-chromogen detection. All the sections were examined under microscope and the immunoreactivity was judged by dark brown staining. (A) Representative picture of stained gastric normal adjacent tissues showing no visible MUC4 staining. (B) Representative picture of gastric adenocarcinoma tissues showing diffused MUC4 staining. All sections were counter stained with haematoxylin. In all the top panels, original magnification is × 10 and in bottom panels, original magnification is × 40.