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. 2008 Sep 9;99(6):949–956. doi: 10.1038/sj.bjc.6604632

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Copyright © 2008 Cancer Research UK

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Expression of MUC4 in AGS and its derived sublines: AGS-vector (empty vector transfected) and AGS-MUC4 (MUC4-transfected) cells. (A) Western blot analysis: total protein lysates were prepared from the subconfluent cells. A total of 20 μg protein from cell extracts was electrophoretically resolved on 2% Agarose gel. Resolved proteins were transferred onto PVDF membrane and probed with MUC4 MAb (8G7). Protein from FG (pancreatic cancer cell line) cells was taken as a positive control. (B) Expression analysis of MUC4 using confocal microscopy: Cells were grown at a low density on sterilised cover slips; after methanol fixation, slides were incubated with MUC4 MAb (8G7), followed by FITC-conjugated secondary antibody, and were observed under a ZEISS confocal laser-scanning microscope (magnification, × 630).