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. 1988 Nov;62(11):4387–4392. doi: 10.1128/jvi.62.11.4387-4392.1988

Vesicular stomatitis virus M protein in the nuclei of infected cells.

D S Lyles 1, L Puddington 1, B J McCreedy Jr 1
PMCID: PMC253880  PMID: 2845149

Abstract

The M protein of vesicular stomatitis virus (VSV) was localized in the nuclei and cytoplasm of VSV-infected cells by subcellular fractionation and immunofluorescence microscopy. Nuclei isolated from VSV-infected Friend erythroleukemia cells were fractionated into a nuclear membrane and a nucleoplasm fraction by DNase digestion and differential centrifugation. G protein was present in the membrane fraction, and M protein was present in the nucleoplasm fraction. Immunofluorescence detection of M protein in the nucleus required that fixed cells be permeabilized with higher concentrations of detergent than were required for detection of M protein in the cytoplasm of VSV-infected BHK cells.

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Selected References

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