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. 2008 Jun 12;586(Pt 14):3537–3550. doi: 10.1113/jphysiol.2008.153577

Figure 1. Induction of N-terminal truncated cardiac TnT by regional ischaemia reperfusion.

Figure 1

Regional ischaemia reperfusion was produced in isolated rat working hearts by reversible ligation of the left anterior descending (LAD) coronary artery. A, the SDS-PAGE and Western blots using anti-TnT mAb CT3 on total myocardial protein extracts from perfused control, ischaemia and ischaemia–reperfusion groups demonstrated that the ischaemic area had significant production of cTnT-ND. In the ischaemia-reperfused hearts, cTnT-ND was also produced in remote zones of the myocardia, indicating an ex vivo whole organ response. B, quantification of the Western blots showed that the levels of cTnT-ND versus total cTnT increased in the left ventricular ischaemic area after LAD ligation and in both left and right ventricles after LAD ischaemia–reperfusion (I-R). n = 3 in each group. **P < 0.01 versus perfused controls; ##P < 0.01 versus right ventricle after LAD ischaemia.