Figure 10. Effect of glutamate receptor blockade on Ca²⁺ influx and DCD occurrence in isolated RGCs.

A, mean Ca²⁺ influx (peak fura-4F ratio over first 400 s of glutamate exposure minus baseline ratio; +1 s.d.) induced by 100 μm glutamate (plus 10 μm glycine) in the presence or absence of AMPA/kainate-R (NBQX) and NMDA-R (Mg²⁺, APV and MK-801) blockers. The data for 100 μm glutamate (blockers absent) is re-plotted from data shown in Fig. 9. B, the proportion of cells exhibiting DCD in each treatment group. C and D, fura-4F ratio traces of all RGCs (n = 75) treated with 100 μm glutamate plus 10 μm glycine for 1 h (with 15 min wash-out) alone (C), and in the presence of the non-competitive NMDA-R antagonist MK-801 (n = 40) (D). RGCs exhibiting DCD are denoted by red traces while RGCs surviving the glutamate insult are denoted by blue traces. *P < 0.05, **P < 0.01, as compared to 100 μm glutamate-treated group; Kruskal–Wallis ANOVA, Dunn's post hoc test for Ca²⁺ influx data; Chi-square for DCD incidence data.