Figure 5. Forskolin enhances InsP₃R-1 channel activity.

A, representative sweeps at −100 mV from an individual cell at the indicated time points. The left panel is sweeps before forskolin application, the middle panel is sweeps during forskolin application to activate adenylate cyclase and PKA, and the right panel contains sweeps after wash-out of the forskolin. B, a diary plot of the P_oversus time, demonstrating that activation of adenylate cyclase markedly enhances P_oversus channel activity prior to stimulation (P = 0.0012).