Figure 1. Effect of temperature, conventional K⁺ channel inhibitors (K-Ins) and tetracaine (TET) on resting membrane potential (RMP) in ED11 atrial myocytes.

Chicken ED11 atrial myocytes were perfused in normal Tyrode solution at room temperature (RT; 20 ± 2°C). Membrane potentials were recorded in current-clamp mode after formation of the whole-cell patch-clamp configuration. A, an example record of membrane potentials illustrating increased polarization when raising the bath temperature to 35°C and the effects of inhibitors. B, bar graph illustrating the mean increase in RMP when going from RT to 35°C (n = 9). Note that RMP at RT is an average of oscillating potentials. C, summarized change in RMP after addition of K-Ins and K-Ins plus TET (n = 5). Data were presented as mean ±s.e.m.