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Summary of real-time PCR in chicken ED11 embryonic heart
| Gene | Tissue | PCR efficiency | Threshold cycle | n |
|---|---|---|---|---|
| GAPDH | ED11A | 2.0606 | 16.36 ± 0.06 | 9 |
| ED11V | 16.72 ± 0.07 | 9 | ||
| TREK-1 | ED11A | 1.928 | 29.66 ± 0.12 | 9 |
| ED11A | 31.94 ± 0.18 | 8 | ||
| TREK-2 | ED11A | 1.8516 | 31.57 ± 0.12 | 14 |
| ED11V | 34.72 ± 0.18 | 12 |
