Skip to main content
. 2008 Feb 4;105(6):1919–1924. doi: 10.1073/pnas.0712063105

Fig. 6.

Fig. 6.

Hbo1-WT, but not Hbo1-S57A, rescues Hbo1-depletion-induced phenotypes in HeLa cells. (A) Cells were cotransfected with pSuper-Hbo1 and pBabe-puro at a ratio of 7:1 and selected with puromycin. Nuclear extracts were subjected to Western blot analysis. (B) Cells were cotransfected with pSuper-Hbo1, Flag-Hbo1, or RNAi-resistant Flag-Hbo1 and pBabe-puro at a ratio of 6:3:1 and selected as in A. Nuclear extracts were subjected to Western blot analysis. (C) Cells were prepared as in A and harvested. Lysates were separated into cytoplasmic, soluble nucleoplasmic, and chromatin-enriched fractions and then subjected to Western blot. (D) Cells grown on coverslips were cotransfected with pSuper-Hbo1 (or pSuper) and RNAi-resistant GFP-Hbo1-WT (or -S57A) at a ratio of 2:1. At 3 d after transfection, cells were labeled with BrdU for 30 min, and the GFP-positive cells were counted.