Protein levels (A) and respiration (B). (A) Levels of mt and cytosolic proteins detected by Western blotting in strains of T. brucei, T. equiperdum, and T. evansi. MRP1/2, mtRNA-binding proteins 1/2; TbRGG1, editing-associated RGG1 protein; RBP16, RNA-binding protein 16; TbTUT, terminal uridyl transferase; UMSBP, universal minicircle sequence binding protein; POL IC, mt DNA polymerase IC; apo C1, apocytochrome Cyt C1; Cyt C, cytochrome c; Rieske, Rieske Fe-S protein; trCO4, trypanosome cytochrome oxidase subunit 4; CO6, cytochrome oxidase subunit 6; p18, ATP synthase subunit b; ATPase β, ATP synthase subunit β; hsp60, heat shock protein 60. (B) Relative contribution of TAO-mediated pathway (AP) and cytochrome-mediated pathway (CP) in T. brucei PS and BS, and the Dk/Ak strains. The amount of O2 consumption inhibited by KCN was measured as the CP capacity. The mean and the SD values of three experiments are shown.