Fig. 2.

GATA-2 L359V has enhanced transcriptional activity on target genes. (A) Transfection assays were conducted in 293T cells using a GATA-2 response element-coupled luciferase reporter, with wtGATA-2-, GATA-2 L359V-, or Δ341–346 mutant-expressing plasmid. (B–D) Examination of mRNA levels of EpoR, EDN-1, and P-selectin using real-time RT-PCR in BM samples isolated at either the CP or BC stage of indicated patients (n = 3). (E) Comparison of the binding affinities of three different GATA-2 proteins with CBP or HDAC1 in a GST pull-down assay. (F) Gel-shift assay on the specific binding of the three GATA-2 proteins with a DNA probe containing a classic GATA-2 response element.