Figure 6.

Analysis of the distribution of ER tubule rates in XTC and egg cytosols. (A) Floated egg organelles incubated in XTC cytosol displayed two apparent peaks of of ER extension rates: a slow rate at 0.3–0.5 μm/s, and a faster rate of between 0.7 and 1.8 μm/s. (B) When 20 μM vanadate was included, the faster peak of rates was abolished, but a slower peak of between 0.2 and 0.5 μm/s remained. (C) In egg cytosol, only a single peak of rates of egg ER motility at between 1.0 and 2.0 μm/s was observed (all motility was abolished by vanadate).