Table 1.
Characteristics of microtubule-based ER motility in X. laevis
| Assay description | Directionality summary | Rate (μm/sec) ±SD | ||
|---|---|---|---|---|
| Movement of egg ER in egg cytosol (1st interphase) | Minus end | 100% | (n > 100)a | 1.65 ± 0.29a |
| Plus end | 0% | |||
| Movement of egg ER in activated egg extracts (2nd interphase) | Minus end | 100% | (n = 16) | NDb |
| Plus end | 0% | |||
| Movement of floated egg ER in XTC cytosol | Minus end | 81% | (n = 42) | 1.29 ± 0.30 (n = 26) |
| Plus end | 19% | 0.38 ± 0.05 (n = 6) | ||
| Movement of floated egg ER in XTC cytosol + 20 μM vanadate | Minus end | 0% | (n = 17) | |
| Plus end | 100% | 0.47 ± 0.09 (n = 14) | ||
a
Data from Allan (1995).
b
ND, not determined.