Table 3.

Phenotypes of wild type (RSH/RSH), heterozygotes (RSH/rsh), homozygotes (rsh/rsh), and their transformants

Transgene used to rescue mutant	Wild-type RSH/RSH	RSH/rsh	rsh/rsh
No transgene	Wild type	Wild type	Embryo lethal
RSH	Wild type	Wild type	Wild type
RSH::EGFP (C-terminal)	Range*	Range*	Embryo lethal
EGFP::RSH (N-terminal)	Wild type	Wild type	Wild type

*In 30 independent transformants of each, phenotypes ranged from wild type to severely defective embryo mutants; this could be a consequence of competition between monomers of RSH and C-terminal fusions of RSH in polymer formation. Note that all of the N-terminal RSH fusions rescued the mutant.