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. Author manuscript; available in PMC: 2009 Aug 15.

Published in final edited form as: J Chromatogr A. 2008 Jun 22;1202(1):75–82. doi: 10.1016/j.chroma.2008.06.020

Panel A: Emission spectrum of Ni²⁺-NTA purified GFP-C/EBP in TE 0.4, pH = 7.5, T = 25 °C. Excitation was at 398 nm. The protein concentration was 1 μM in 200 μL volume of buffer. Panel B: Generation of a standard curve GFP-C/EBP in TE 0.4, pH = 7.5, T = 25 °C. Excitation was at 398 nm and emission was monitored at 512 nm. The protein concentrations are in the range 0.7 to 7.5 μg.ml⁻¹. The dashed line is the fit to the equation for a straight line. Panel C: UV-Visible scans of GFP-C/EBP (0.5 μM, solid line), 0.5 μM RS-S-EP36-S-SR′ (solid line) and HS-S-EP36-S-SH (dotted line), TP (5 μM, solid line), and 100 mM DTT (dashed line) in TE0.4, pH=7.5 buffer at 25 °C. The λ_max values observed are in the parentheses for the individual scans.

Panel A: Emission spectrum of Ni²⁺-NTA purified GFP-C/EBP in TE 0.4, pH = 7.5, T = 25 °C. Excitation was at 398 nm. The protein concentration was 1 μM in 200 μL volume of buffer. Panel B: Generation of a standard curve GFP-C/EBP in TE 0.4, pH = 7.5, T = 25 °C. Excitation was at 398 nm and emission was monitored at 512 nm. The protein concentrations are in the range 0.7 to 7.5 μg.ml⁻¹. The dashed line is the fit to the equation for a straight line. Panel C: UV-Visible scans of GFP-C/EBP (0.5 μM, solid line), 0.5 μM RS-S-EP36-S-SR′ (solid line) and HS-S-EP36-S-SH (dotted line), TP (5 μM, solid line), and 100 mM DTT (dashed line) in TE0.4, pH=7.5 buffer at 25 °C. The λ_max values observed are in the parentheses for the individual scans.