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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Chromatogr A. 2008 Jun 22;1202(1):75–82. doi: 10.1016/j.chroma.2008.06.020

Figure 5.

Figure 5

Reaction with iodoacetamide does not affect DNA-binding. Lane 1 is the radiolabeled EP24 DNA (5′-32P-GCTGCAGATTGCGCAATCTGCAGCGTGTGTGTGT-OH-3′) without protein. The gel shifts due to bacterial crude, iodoacetamide treated bacterial crude, purified GFP-C/EBP, and iodoacetamide treated purified GFP-C/EBP are shown in lanes 2, 3, 4, and 5, respectively. In the experiments 2 μL of the purified and crude proteins were used.