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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Chromatogr A. 2008 Jun 22;1202(1):75–82. doi: 10.1016/j.chroma.2008.06.020

Figure 6.

Figure 6

Elution profile of HS-EP36-SH (thiol DNA) and purified Ni2+-NTA purified GFP-C/EBP complex to disulfide column (TP-TPS) in TE0.4, pH=7.5 buffer at 25 °C. TE0.4, 10 mL containing 16 nM HS-EP36-SH and 3.2 nM GFP-C/EBP was incubated on ice for 30 mi. and loaded on a 1 ml TP-TPS column (fractions 1–44). Fractions (0.25 ml) were collected and the fluorescence determined. The column was then washed with 7.5 mL TE 1.2 (fractions 45–74) and then eluted with 100 mM DTT in TE0.1 (fractions 75–89).