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. Author manuscript; available in PMC: 2009 Aug 15.
Published in final edited form as: J Chromatogr A. 2008 Jun 22;1202(1):75–82. doi: 10.1016/j.chroma.2008.06.020

Figure 7.

Figure 7

The elution profile of the GFP-C/EBP from the carboxyamidomethylated bacterial crude and reduced HS-EP36-SH from the TP-TPS column by 100 mM DTT. 0.46 ml of carboxyamidomethylated crude bacterial extract was mixed with 25 ml TE0.4 containing 10.8 nmol of HS-EP36-SH and applied to a 2 ml TP-TPS column. The column was then washed with 10 ml of TE, 8 ml of TE1.2, 9 ml of TE1.6, 10 ml of TE2.0 (data not shown), and eluted with 100 mM DTT in TE0.4. The 200 μL fractions were collected using 96 well plates and the fluorescence emission intensities were monitored at 512 nm by exciting the samples at 398 nm. The fractions from loading and washing are not shown as they do not have any fluorescence (see Results). Elution with 100 mM DTT in TE0.4 begins at fraction 1 for the plate shown.