. Author manuscript; available in PMC: 2009 Aug 15.

Published in final edited form as: J Chromatogr A. 2008 Jun 22;1202(1):75–82. doi: 10.1016/j.chroma.2008.06.020

TABLE 1.

Elution of HS-EP36-SH (thiol DNA) bound to disulfide (MNB-TPS) column.

Fraction number	Absorbance of MNB at 412 nm (A.U.)	Ellipticity from circular dichroism (mdeg.cm⁻¹)
1	0.01	not seen
2	0.31	0.67
3	0.05	not seen
4	0.05	not seen
5	not seen	not seen
6	not seen	not seen
7	not seen	not seen
8	not seen	not seen
9	not seen	not seen
10	not seen	5.43
11	not seen	not seen

The column volume was made of 1 mL of MNB-TPS beads. The column was loaded with 200 μl of 54.2 μM HS-EP36-HS DNA and washed with 10 ml 0.5 M sodium phosphate, pH=7.2, (fractions 1–9) and then eluted with 100 mM DTT in the same buffer. Fractions were 1 ml. Absorbance at 412 nm was measured using a Tecan plate reader and circular dichroism (ellipticity) was also measured. The data for all fractions for both measurements is shown but some are close to baseline in the scans and are reported as ‘not seen’.