Table 1.
Experimental isotope ratios for ten fatty acids in a simulated relative quantification experiment where the expected ratio was 4.00
| Fatty Acida | Experimental Ratiob | Precision CV (%) | Accuracy|% error| |
|---|---|---|---|
| 16:0 | 4.21 | 3.1 | 5.4 |
| 16:1 | 4.04 | 0.8 | 1.1 |
| 18:0 | 4.30 | 2.3 | 7.5 |
| 18:1 | 4.22 | 2.6 | 5.5 |
| 18:2 | 4.29 | 1.4 | 7.4 |
| 18:3 | 3.98 | 6.3 | 0.6 |
| 20:3 | 3.50 | 4.8 | 12.5 |
| 20:4 | 4.15 | 1.8 | 3.7 |
| 22:5 | 4.04 | 1.7 | 1.0 |
| 22:6 | 3.94 | 1.3 | 1.4 |
| Average | 4.07 | 2.6 | 4.6 |
a
Fatty acids are referred to by their number of carbons and degree of unsaturation.
b
A 1:1 mixture was used to normalize ratios for minor variations in reactivity between heavy and light cholamine.