Figure 3.

Mitochondrial DNA replication in axonal mitochondria after disruption of anterograde traffic by MT depletion. DRG cultures were grown overnight and then treated with vinblastine for 3 h. They were then grown for an additional 3 h in the absence (A–C) or presence of BrdU (D–F) and finally assayed by immunocytochemistry. The phase contrast (A,D), epifluorescence images of MitoTracker (B,E), and BrdU (C,F) staining are shown earlier. BrdU signals were detected along the axon of vinblastine-treated cells (F). No background signal was observed in the control cells which had not been labeled with BrdU (C). Scale bar, 10 μm.