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. Author manuscript; available in PMC: 2008 Sep 17.
Published in final edited form as: J Immunother. 1997 Jan;20(1):15–25. doi: 10.1097/00002371-199701000-00002

TABLE 3.

Recognition of mouse MART1 by human MART1-reactive tumor-infiltrating lymphocytes (TILs)

IFNγ secretion (pg/ml/105 cells)a
Stimulator Transfccted gene(s) HLA-A2 TIL620b TIL1200 TIL1235
Cos7 none - 0 0 0
Cos7 HLA-A2 + 12 73 46
Cos7 hMART1 - 0 99 28
Cos7 mMART1 - 0 62 43
Cos7 hMART1 + HLA-A2 + 112 90 460
Cos7 mMART1 + HLA-A2 + 168 83 552
SK-23c + 1,702 1,712 1,663
B16 - 0 10 54
a

Human interferon-γ (IFNγ) concentration was determined by enzyme-linked immunosorbent assay after TILs were coincubated for 24 h with stimulators with or without transiently transfected genes indicated.

b

TIL620 and TIL235 are HLA-A2-restricted cytotoxic T-lymphocytes that recognize the MART1 antigen, whereas TIL1200 recognizcs human gp100 specifically.

c

SK-23 and B16 are melanoma tumor lines used as positive and negative controls, respectively.