TABLE 3.
IFNγ secretion (pg/ml/105 cells)a |
|||||
---|---|---|---|---|---|
Stimulator | Transfccted gene(s) | HLA-A2 | TIL620b | TIL1200 | TIL1235 |
Cos7 | none | - | 0 | 0 | 0 |
Cos7 | HLA-A2 | + | 12 | 73 | 46 |
Cos7 | hMART1 | - | 0 | 99 | 28 |
Cos7 | mMART1 | - | 0 | 62 | 43 |
Cos7 | hMART1 + HLA-A2 | + | 112 | 90 | 460 |
Cos7 | mMART1 + HLA-A2 | + | 168 | 83 | 552 |
SK-23c | + | 1,702 | 1,712 | 1,663 | |
B16 | - | 0 | 10 | 54 |
Human interferon-γ (IFNγ) concentration was determined by enzyme-linked immunosorbent assay after TILs were coincubated for 24 h with stimulators with or without transiently transfected genes indicated.
TIL620 and TIL235 are HLA-A2-restricted cytotoxic T-lymphocytes that recognize the MART1 antigen, whereas TIL1200 recognizcs human gp100 specifically.
SK-23 and B16 are melanoma tumor lines used as positive and negative controls, respectively.