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. Author manuscript; available in PMC: 2008 Sep 17.
Published in final edited form as: Anal Chem. 2007 Apr 6;79(10):3623–3634. doi: 10.1021/ac062292d

Figure 2. Improved peptide recovery from ion-exchanger resins – chromatographic comparison between SCX and ACE mixed-bed formats.

Figure 2

Typical base peak chromatograms and MS/MS spectra from triplicate recovery experiments are shown. Tryptic digests of a five protein standard mixture were eluted from each ion-exchanger column using a 1-step high-concentration salt pulse and subsequently analyzed by online reversed-phase gradient LC/MS/MS. The majority of peptides observed in the ACE mixed-bed platform (B) had greater intensities than those of the SCX format (A). The amounts of packed SCX resin were adjusted to be identical for both cases to make the comparison valid (i.e., the SCX bed was 1/3 length of the WAX+SCX(2:1) bed).