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Gel filtration chromatography. Wild-type (A) and erp1/erp5/erp6 mutant (B) yeast cells were converted to spheroplasts and lysed, and the cleared extract was applied to a Superose 6 HR 10/30 gel filtration column. The fractionation was calibrated by parallel runs of molecular weight markers. Individual p24 proteins were detected by Western blot analysis.
