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Invertase secretion in wild-type and p24 mutant cells. Exponentially dividing cells were transferred to low-glucose media for 1 h to induce invertase synthesis. Spheroplasts were prepared and pelleted by centrifugation to obtain the cytoplasmic (I) and periplasmic (E) fractions. Invertase was detected by an in-gel activity assay. The cytoplasmic, core, and hyperglycosylated forms of invertase are indicated.
