Figure 3.
Maturation of Gas1p in wild-type and p24 deletion strains. (A) Whole-cell extracts were prepared from exponentially growing cells, separated by SDS-PAGE, and detected by Western blotting using an anti-Gas1p antibody. Migration of mature (mGas1p) and precursor (pGas1p) forms of Gas1p are indicated. (B) Extracts were prepared from wild-type, Δemp24, Δerv25, and Δerp1 cells after a 5-min pulse with 35S-labeled amino acids followed by chase for the indicated times. Gas1p was precipitated from the extracts, and immune complexes were precipitated, separated by SDS-PAGE, and detected by autoradiography. Migration of mature (mGas1p) and precursor (pGas1p) forms of Gas1p is indicated.