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Genetic redundancy among p24 genes. Individual p24 genes were overexpressed from the TPI promotor in erp1 or erp2 mutant cells grown at 30°C in YPD. Exponentially dividing cells were washed and resuspended in fresh medium. Proteins of the culture medium of 0.3 OD600 unit equivalents were TCA precipitated after 4.5 h and resolved by SDS-PAGE. Kar2p was detected by Western blotting with anti-Kar2p antiserum.
