Figure 9.

Immunoprecipitation of Erp1p from radiolabeled wild-type and erp1/5/6 mutant cells. Erp1p was precipitated from solubilized extracts prepared from radiolabeled wild-type or erp1/5/6 mutant cells (A) or unlabeled cells (B) as described in MATERIALS AND METHODS. Immunoprecipitates were resolved on 12% SDS-polyacrylamide gels and either dried and exposed to film (A) or transferred to nitrocellulose (B) and probed with antibodies against Erp1p (lanes 3 and 4), Erp2p (lanes 5 and 6), Erv25p (lanes 7 and 8), and Emp24p (lanes 9 and 10). Immunoprecipitates in lanes 1, 3, 5, 7, and 9 were prepared from wild-type cells; those in lanes 2, 4, 6, 8, and 10 were prepared from erp1/5/6 mutant cells. The immunoreactive protein detected at ∼48 kDa corresponds to the heavy chain of the anti-Erp1p antibodies used for the immunoprecipitation.